Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer

Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases.     

东海盆地油气勘探焦点问题探讨

东海盆地自西向东可分为 3个带 ,即西部断陷带、中部隆起带和东部断坳带 ,其构造格局各具特色 ,并且均有自身的烃源岩。西部断陷带由多个以古新世为发育高峰期的半地堑组成 ,主要烃源岩为下古新统湖相泥岩 ,该带有利的勘探区是丽水、椒江凹陷 ,围绕下古新统主力烃源岩寻找油气是丽水、椒江凹陷油气勘探的焦点 ;中部隆起带是中生界残留盆地的叠置区 ,主要烃源岩为侏罗系湖相泥岩 ,福州凹陷是该带较有利的勘探区 ,烃源岩的规模、潜力以及圈闭保存条件是福州凹陷油气勘探的焦点 ;东部断坳带是厚度逾万米的新生代沉积断坳区 ,主要烃源岩为始新世煤系地层 ,该带有利的勘探区是西湖凹陷 ,烃源岩和盖层条件是西湖凹陷油气勘探的关键。目前 ,东海盆地比较现实和最具勘探前景的地区是西湖凹陷及丽水、椒江凹陷     

地层岩性随钻识别的神经网络方法研究

钻井过程中随钻识别钻头当前位置的岩性信息对于合理选择钻头类型、快速建立岩性剖面、及时发现油气层和卡准取心层位有着重要意义。钻井实践证明，地层岩性的变化在钻井参数上有很好的综合体现。以录井资料为基础，结合已钻井的测井资料，根据BP神经网络原理，建立了地层岩性随钻识别神经网络模型。应用该模型在新疆油田进行了地层岩性随钻识别试验，试验结果与测井资料解释结果相比，效果较好，符合率可达80％。     

松辽盆地南部深层火山岩识别及成藏条件分析

松辽盆地北部徐深1井在营城组火山岩获得油气突破后，在松辽盆地南部长岭断陷针对火山岩部署了长深1井。在长深1井营城组火山岩地层测试获4.6×105m3/d的工业气流，推算无阻流量1.5×106m3/d以上，预示松辽盆地南部深层火山岩气藏是下步勘探开发的主要领域。通过大量的火山岩岩心资料，利用岩石学、矿物学、测井和地震地层学等多学科技术，首次在松辽盆地南部建立了火山岩识别标准，在东南隆起区部署探井1口，在火山岩中见到了较好的气显示，取得很好的效果。     

致密低渗气藏储层应力敏感性及其对单井产能的影响

采用变出口端回压应力敏感实验方法，具体实测了大牛地致密低渗气藏储集层岩样不同驱替压差下的渗透率，根据实测的结果回归出大牛地致密低渗气藏储集层应力敏感性的幂函数关系式，总结了储层渗透率随气藏压力的变化规律。在考虑储集层应力敏感影响的情况下，推导出新的产能方程，计算了渗透率变化对单井产能的影响。研究表明：大牛地致密低渗气藏储集层应力敏感性在30％-70％之间，在考虑储集层应力敏感性的条件下，气藏单井产能降低，平均绝对无阻流量是渗透率为常数时的76．49％。     

A three‐dimensional model describing stress‐temperature induced solid phase transformations: solution algorithm and boundary value problems

An always increasing knowledge on material properties as well as a progressively more sophisticated production technology make shape memory alloys (SMA) extremely interesting for the industrial world. At the same time, SMA devices are typically characterized by complex multi‐axial stress states as well as non‐homogeneous and non‐isothermal conditions both in space and time. This aspect suggests the finite element method as a useful tool to help and improve application design and realization. With this aim, we focus on a three‐dimensional macroscopic thermo‐mechanical model able to reproduce the most significant SMA features (Int. J. Numer. Methods Eng. 2002; 55 : 1255–1264), proposing a simple modification of such a model. However, the suggested modification allows the development of a time‐discrete solution algorithm, which is more effective and robust than the one previously discussed in the literature. We verify the computational tool ability to simulate realistic mechanical boundary value problems with prescribed temperature dependence, studying three SMA applications: a spring actuator, a self‐expanding stent, a coupling device for vacuum tightness. The effectiveness of the model to solve thermo‐mechanical coupled problems will be discussed in a forthcoming work. Copyright © 2004 John Wiley & Sons, Ltd.     

中国碎屑岩隐蔽油气藏勘探进展与问题

介绍了中国陆上与海上碎屑岩隐蔽油气藏的勘探历程.总结了伴随碎屑岩隐蔽油气藏勘探过程中新理论新技术的进展情况,理论上包括层序地层理论在中国的不断创新、坡折带理论、复式输导体系、相势控藏及三元控藏理论,这些理论推动了隐蔽油气藏从个别识别到定性和定量预测,促进了盆地不同构造位置、不同层系、不同领域隐蔽油气藏的勘探;技术上主要体现在高精度层序地层研究、为高精度层序地层研究而发展的保幅处理和高分辨率处理技术、验证和预测隐蔽砂体的反演技术、隐蔽砂体快速直观识别的可视化技术、分析隐蔽砂体含油性的AVO技术及分频等地球物理技术.尽管如此,隐蔽油气藏勘探失败案例暴露了许多问题,这些问题在于缺乏系统运用隐蔽油气藏理论或者由于隐蔽油气藏成藏理论不完善难以准确快速评价是否成藏,此外,由于地球物理预测具有多解性也会导致错误的认识.因此,今后应充分利用高分辨率地震资料,大力推进叠前处理和反演技术,进一步弄清隐蔽油气藏成藏机理及主控因素.     

富CO2流体对深部碎屑储层影响机制模拟研究

深部流体中富含CO2,进入沉积盆地之后可与碎屑岩储集层相互作用,改变储层物性.利用压实成岩模拟装置,采用粒径为0.3～0.5 mm的长石颗粒为研究对象,开展了注入CO2的模拟试验,并通过分析流体中的化学成分变化监测发生的化学反应.注入CO2后取样表明,Si、Ca、Na的离子浓度在注入CO2后28h轻微下降;但在注入67h后Si和Na离子浓度上升,Ca离子浓度下降;注入150h后的取样表明,Ca离子浓度升高.加入CO2后试验砂体的孔隙度和渗透率下降,但渗透率降低的速率更快.表明CO2进入沉积盆地的初期,由于形成CaCO3沉淀和钙蒙脱石等新矿物,砂体渗透率降低,储层物性变差.     

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本文提出了凹型压力恢复曲线的一阶导数图版和二阶导数图版。综合使用这两种导数图版,提高了匹配的精度,使匹配结果准确。无量纲起始压力梯度D很难确定。本文提出的两种导数图版综合匹配法能准确确定D值,是致密储层动态评价的有效工具。